Exceptional Selectivity of Agilent ZORBAX Eclipse Plus Phenyl-Hexyl Columns to Separate Estrogens

A new ZORBAX stationary phase, Agilent ZORBAX Eclipse Plus Phenyl-Hexyl, separated five estrogen type steroids better than a C18 and better than three other phenyl columns under identical conditions, due to its unique selectivity for analytes containing phenyl groups. The comparisons support selectivity as the most important factor to optimize resolution, to separate peaks completely. Selectivity can be quickly altered by simply substituting columns with different phases; therefore, having a variety of columns (stationary phases) available for method development improves the chances of discovering the best HPLC method. Eclipse Plus Phenyl-Hexyl characteristics such as improved silica, bonding technology, and molecular interactions of the phenyl-hexyl moiety with aromatic groups of the analytes make the Eclipse Plus Phenyl-Hexyl column valuable for a method developer's column collection. Authors John W. Henderson Jr. and William J. Long Agilent Technologies, Inc. 2850 Centerville Road Wilmington, DE 19809-1610 USA Application Note Pharmaceutical, Environmental